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ov90 human serous ovarian cancer cell lines  (ATCC)


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    ATCC ov90 human serous ovarian cancer cell lines
    Carboplatin-resistant ovarian cancer exhibited rewired oxidize redox state “high level ROS while low level antioxidants”. (a) Intracellular peroxides levels measured by DCF fluorescence probe. PEG-CAT (500 units) were added 24 hr prior to the assay. (b) Extracellular H 2 O 2 levels in the media after 24-hr culturing. Amplex Red was used for the assay. (c) Intracellular of H 2 O 2 in picomole after 24-h culturing. (d) Western blot assay for the steady-state level of antioxidant proteins. Lysates were collected after 24-hr culturing. (e) Antioxidant activities. Cells were collected after 24-hr culturing and sonicated for lysates. MnSOD activity was measured using activity gel with NBT. GPx activity was assessed using a commercial kit. Oxidized form of Trx1 was measured with redox western blot assay. ∗ P ≤ .05 versus hTER7 group. # P ≤ .05 versus <t>OV90</t> group.
    Ov90 Human Serous Ovarian Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 416 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ov90+human+serous+ovarian+cancer+cell+lines/pmc09313984-47-0-10?v=ATCC
    Average 96 stars, based on 416 article reviews
    ov90 human serous ovarian cancer cell lines - by Bioz Stars, 2026-07
    96/100 stars

    Images

    1) Product Images from "A Redox-active Mn Porphyrin, MnTnBuOE-2-PyP 5+ , Synergizes with Carboplatin in Treatment of Chemoresistant Ovarian Cell Line"

    Article Title: A Redox-active Mn Porphyrin, MnTnBuOE-2-PyP 5+ , Synergizes with Carboplatin in Treatment of Chemoresistant Ovarian Cell Line

    Journal: Oxidative Medicine and Cellular Longevity

    doi: 10.1155/2022/9664636

    Carboplatin-resistant ovarian cancer exhibited rewired oxidize redox state “high level ROS while low level antioxidants”. (a) Intracellular peroxides levels measured by DCF fluorescence probe. PEG-CAT (500 units) were added 24 hr prior to the assay. (b) Extracellular H 2 O 2 levels in the media after 24-hr culturing. Amplex Red was used for the assay. (c) Intracellular of H 2 O 2 in picomole after 24-h culturing. (d) Western blot assay for the steady-state level of antioxidant proteins. Lysates were collected after 24-hr culturing. (e) Antioxidant activities. Cells were collected after 24-hr culturing and sonicated for lysates. MnSOD activity was measured using activity gel with NBT. GPx activity was assessed using a commercial kit. Oxidized form of Trx1 was measured with redox western blot assay. ∗ P ≤ .05 versus hTER7 group. # P ≤ .05 versus OV90 group.
    Figure Legend Snippet: Carboplatin-resistant ovarian cancer exhibited rewired oxidize redox state “high level ROS while low level antioxidants”. (a) Intracellular peroxides levels measured by DCF fluorescence probe. PEG-CAT (500 units) were added 24 hr prior to the assay. (b) Extracellular H 2 O 2 levels in the media after 24-hr culturing. Amplex Red was used for the assay. (c) Intracellular of H 2 O 2 in picomole after 24-h culturing. (d) Western blot assay for the steady-state level of antioxidant proteins. Lysates were collected after 24-hr culturing. (e) Antioxidant activities. Cells were collected after 24-hr culturing and sonicated for lysates. MnSOD activity was measured using activity gel with NBT. GPx activity was assessed using a commercial kit. Oxidized form of Trx1 was measured with redox western blot assay. ∗ P ≤ .05 versus hTER7 group. # P ≤ .05 versus OV90 group.

    Techniques Used: Fluorescence, Western Blot, Sonication, Activity Assay

    Increased mitochondrial respiratory and glycolytic activity are linked with carboplatin-resistant cancer. (a) Parameters of mitochondrial function calculated from OCR. (b) Glycolytic activity calculated from ECAR of live OV90 cells and OVCD cells. # P ≤ .05 versus OV90 group.
    Figure Legend Snippet: Increased mitochondrial respiratory and glycolytic activity are linked with carboplatin-resistant cancer. (a) Parameters of mitochondrial function calculated from OCR. (b) Glycolytic activity calculated from ECAR of live OV90 cells and OVCD cells. # P ≤ .05 versus OV90 group.

    Techniques Used: Activity Assay

    MnP sensitized resistant ovarian cancer to carboplatin while protecting normal cells from chemotherapy-induced injury. Cells were cultured for 24 prior to treatment with 50 nM MnP and/or CBCD (7 μ M). (a) OV90 cell viability and (b) OVCD cell viability after treatment of MnP and/or CBCD (24 hr). (c) hTER7 cell viability after treatment of MnP and/or CBCD, CDDP, PTX (24 hr). % Cell viability was normalized to no-treatment. ∗ P ≤ .05 versus hTER7 group. ∗∗ P ≤ .05 versus CBDCA alone.
    Figure Legend Snippet: MnP sensitized resistant ovarian cancer to carboplatin while protecting normal cells from chemotherapy-induced injury. Cells were cultured for 24 prior to treatment with 50 nM MnP and/or CBCD (7 μ M). (a) OV90 cell viability and (b) OVCD cell viability after treatment of MnP and/or CBCD (24 hr). (c) hTER7 cell viability after treatment of MnP and/or CBCD, CDDP, PTX (24 hr). % Cell viability was normalized to no-treatment. ∗ P ≤ .05 versus hTER7 group. ∗∗ P ≤ .05 versus CBDCA alone.

    Techniques Used: Cell Culture

    MnP and carboplatin concentration analysis using LC/MS after 1-hr treatment.
    Figure Legend Snippet: MnP and carboplatin concentration analysis using LC/MS after 1-hr treatment.

    Techniques Used: Concentration Assay

    Increasing oxidative stress in cancer by cotreatment of MnP and carboplatin. (a) Intracellular peroxide levels measured by DCF fluorescence probe after 2-hr treatment. PEG-CAT (500 units) were added 24 hr prior to the assay. (b) Intracellular H 2 O 2 levels after 2-hr treatment. (c) Catalase activity after 2-hr treatment. (d) GSH levels were measured by HPLC after 24-hr treatment. (e) MnSOD activity was measured using activity gel with NBT, after 24-hr treatment. (f) GPx activity was determined using a commercial kit after 24-hr treatment. ∗ P ≤ .05 versus hTER7 group. ∗ P ≤ .05 versus no treatment group. # P ≤ .05 versus OV90 group.
    Figure Legend Snippet: Increasing oxidative stress in cancer by cotreatment of MnP and carboplatin. (a) Intracellular peroxide levels measured by DCF fluorescence probe after 2-hr treatment. PEG-CAT (500 units) were added 24 hr prior to the assay. (b) Intracellular H 2 O 2 levels after 2-hr treatment. (c) Catalase activity after 2-hr treatment. (d) GSH levels were measured by HPLC after 24-hr treatment. (e) MnSOD activity was measured using activity gel with NBT, after 24-hr treatment. (f) GPx activity was determined using a commercial kit after 24-hr treatment. ∗ P ≤ .05 versus hTER7 group. ∗ P ≤ .05 versus no treatment group. # P ≤ .05 versus OV90 group.

    Techniques Used: Fluorescence, Activity Assay

    Cotreatment of MnP and carboplatin inhibited mitochondrial respiratory and ATP production in chemoresistant ovarian cancer cells. MnP and/or CBDCA were added to the cells for 6 hr. Live cells were then used for the measurement of OCR and ECAR in OV90 cells (a and c) and in OVCD cells (b and d). (e) Absolute ATP concentration was measured using ATP kits after 24-hr treatment. ∗ P ≤ .05 versus control.
    Figure Legend Snippet: Cotreatment of MnP and carboplatin inhibited mitochondrial respiratory and ATP production in chemoresistant ovarian cancer cells. MnP and/or CBDCA were added to the cells for 6 hr. Live cells were then used for the measurement of OCR and ECAR in OV90 cells (a and c) and in OVCD cells (b and d). (e) Absolute ATP concentration was measured using ATP kits after 24-hr treatment. ∗ P ≤ .05 versus control.

    Techniques Used: Concentration Assay, Control

    MnP-mediated oxidative stress inhibited Nrf2 and its downstream target, Trx1 in ovarian cancer carboplatin-resistant cell but not in a normal cell. Cells were cultured for 24 hr prior to MnP treatment (6 hr). Cells were then collected, and RNA was isolated for RT-PCR. (a) Nrf2 and its downstream targets were compared between hTER7, OV90, and OVCD cells. Nrf2 and its downstream targets in (b) hTER7 cell line, (c) OV90 cell line, and (d) OVCD cell line, after MnP treatment. (e) Immunoprecipitation (IP) is performed with antibody to Trx1 and western blot analysis with anti-Keap1, Nrf2, and Trx1 antibodies were subsequently performed. ∗ P ≤ .05 when compared to control.
    Figure Legend Snippet: MnP-mediated oxidative stress inhibited Nrf2 and its downstream target, Trx1 in ovarian cancer carboplatin-resistant cell but not in a normal cell. Cells were cultured for 24 hr prior to MnP treatment (6 hr). Cells were then collected, and RNA was isolated for RT-PCR. (a) Nrf2 and its downstream targets were compared between hTER7, OV90, and OVCD cells. Nrf2 and its downstream targets in (b) hTER7 cell line, (c) OV90 cell line, and (d) OVCD cell line, after MnP treatment. (e) Immunoprecipitation (IP) is performed with antibody to Trx1 and western blot analysis with anti-Keap1, Nrf2, and Trx1 antibodies were subsequently performed. ∗ P ≤ .05 when compared to control.

    Techniques Used: Cell Culture, Isolation, Reverse Transcription Polymerase Chain Reaction, Immunoprecipitation, Western Blot, Control



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    ov90 human serous ovarian cancer cell lines  (ATCC)
    96
    ATCC ov90 human serous ovarian cancer cell lines
    Carboplatin-resistant ovarian cancer exhibited rewired oxidize redox state “high level ROS while low level antioxidants”. (a) Intracellular peroxides levels measured by DCF fluorescence probe. PEG-CAT (500 units) were added 24 hr prior to the assay. (b) Extracellular H 2 O 2 levels in the media after 24-hr culturing. Amplex Red was used for the assay. (c) Intracellular of H 2 O 2 in picomole after 24-h culturing. (d) Western blot assay for the steady-state level of antioxidant proteins. Lysates were collected after 24-hr culturing. (e) Antioxidant activities. Cells were collected after 24-hr culturing and sonicated for lysates. MnSOD activity was measured using activity gel with NBT. GPx activity was assessed using a commercial kit. Oxidized form of Trx1 was measured with redox western blot assay. ∗ P ≤ .05 versus hTER7 group. # P ≤ .05 versus <t>OV90</t> group.
    Ov90 Human Serous Ovarian Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ov90+human+serous+ovarian+cancer+cell+lines/pmc09313984-47-0-10?v=ATCC
    Average 96 stars, based on 1 article reviews
    ov90 human serous ovarian cancer cell lines - by Bioz Stars, 2026-07
    96/100 stars
    		  Buy from Supplier

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    Carboplatin-resistant ovarian cancer exhibited rewired oxidize redox state “high level ROS while low level antioxidants”. (a) Intracellular peroxides levels measured by DCF fluorescence probe. PEG-CAT (500 units) were added 24 hr prior to the assay. (b) Extracellular H 2 O 2 levels in the media after 24-hr culturing. Amplex Red was used for the assay. (c) Intracellular of H 2 O 2 in picomole after 24-h culturing. (d) Western blot assay for the steady-state level of antioxidant proteins. Lysates were collected after 24-hr culturing. (e) Antioxidant activities. Cells were collected after 24-hr culturing and sonicated for lysates. MnSOD activity was measured using activity gel with NBT. GPx activity was assessed using a commercial kit. Oxidized form of Trx1 was measured with redox western blot assay. ∗ P ≤ .05 versus hTER7 group. # P ≤ .05 versus OV90 group.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: A Redox-active Mn Porphyrin, MnTnBuOE-2-PyP 5+ , Synergizes with Carboplatin in Treatment of Chemoresistant Ovarian Cell Line

    doi: 10.1155/2022/9664636

    Figure Lengend Snippet: Carboplatin-resistant ovarian cancer exhibited rewired oxidize redox state “high level ROS while low level antioxidants”. (a) Intracellular peroxides levels measured by DCF fluorescence probe. PEG-CAT (500 units) were added 24 hr prior to the assay. (b) Extracellular H 2 O 2 levels in the media after 24-hr culturing. Amplex Red was used for the assay. (c) Intracellular of H 2 O 2 in picomole after 24-h culturing. (d) Western blot assay for the steady-state level of antioxidant proteins. Lysates were collected after 24-hr culturing. (e) Antioxidant activities. Cells were collected after 24-hr culturing and sonicated for lysates. MnSOD activity was measured using activity gel with NBT. GPx activity was assessed using a commercial kit. Oxidized form of Trx1 was measured with redox western blot assay. ∗ P ≤ .05 versus hTER7 group. # P ≤ .05 versus OV90 group.

    Article Snippet: OV90 human serous ovarian cancer cell lines were purchased from ATCC.

    Techniques: Fluorescence, Western Blot, Sonication, Activity Assay

    Increased mitochondrial respiratory and glycolytic activity are linked with carboplatin-resistant cancer. (a) Parameters of mitochondrial function calculated from OCR. (b) Glycolytic activity calculated from ECAR of live OV90 cells and OVCD cells. # P ≤ .05 versus OV90 group.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: A Redox-active Mn Porphyrin, MnTnBuOE-2-PyP 5+ , Synergizes with Carboplatin in Treatment of Chemoresistant Ovarian Cell Line

    doi: 10.1155/2022/9664636

    Figure Lengend Snippet: Increased mitochondrial respiratory and glycolytic activity are linked with carboplatin-resistant cancer. (a) Parameters of mitochondrial function calculated from OCR. (b) Glycolytic activity calculated from ECAR of live OV90 cells and OVCD cells. # P ≤ .05 versus OV90 group.

    Article Snippet: OV90 human serous ovarian cancer cell lines were purchased from ATCC.

    Techniques: Activity Assay

    MnP sensitized resistant ovarian cancer to carboplatin while protecting normal cells from chemotherapy-induced injury. Cells were cultured for 24 prior to treatment with 50 nM MnP and/or CBCD (7 μ M). (a) OV90 cell viability and (b) OVCD cell viability after treatment of MnP and/or CBCD (24 hr). (c) hTER7 cell viability after treatment of MnP and/or CBCD, CDDP, PTX (24 hr). % Cell viability was normalized to no-treatment. ∗ P ≤ .05 versus hTER7 group. ∗∗ P ≤ .05 versus CBDCA alone.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: A Redox-active Mn Porphyrin, MnTnBuOE-2-PyP 5+ , Synergizes with Carboplatin in Treatment of Chemoresistant Ovarian Cell Line

    doi: 10.1155/2022/9664636

    Figure Lengend Snippet: MnP sensitized resistant ovarian cancer to carboplatin while protecting normal cells from chemotherapy-induced injury. Cells were cultured for 24 prior to treatment with 50 nM MnP and/or CBCD (7 μ M). (a) OV90 cell viability and (b) OVCD cell viability after treatment of MnP and/or CBCD (24 hr). (c) hTER7 cell viability after treatment of MnP and/or CBCD, CDDP, PTX (24 hr). % Cell viability was normalized to no-treatment. ∗ P ≤ .05 versus hTER7 group. ∗∗ P ≤ .05 versus CBDCA alone.

    Article Snippet: OV90 human serous ovarian cancer cell lines were purchased from ATCC.

    Techniques: Cell Culture

    MnP and carboplatin concentration analysis using LC/MS after 1-hr treatment.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: A Redox-active Mn Porphyrin, MnTnBuOE-2-PyP 5+ , Synergizes with Carboplatin in Treatment of Chemoresistant Ovarian Cell Line

    doi: 10.1155/2022/9664636

    Figure Lengend Snippet: MnP and carboplatin concentration analysis using LC/MS after 1-hr treatment.

    Article Snippet: OV90 human serous ovarian cancer cell lines were purchased from ATCC.

    Techniques: Concentration Assay

    Increasing oxidative stress in cancer by cotreatment of MnP and carboplatin. (a) Intracellular peroxide levels measured by DCF fluorescence probe after 2-hr treatment. PEG-CAT (500 units) were added 24 hr prior to the assay. (b) Intracellular H 2 O 2 levels after 2-hr treatment. (c) Catalase activity after 2-hr treatment. (d) GSH levels were measured by HPLC after 24-hr treatment. (e) MnSOD activity was measured using activity gel with NBT, after 24-hr treatment. (f) GPx activity was determined using a commercial kit after 24-hr treatment. ∗ P ≤ .05 versus hTER7 group. ∗ P ≤ .05 versus no treatment group. # P ≤ .05 versus OV90 group.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: A Redox-active Mn Porphyrin, MnTnBuOE-2-PyP 5+ , Synergizes with Carboplatin in Treatment of Chemoresistant Ovarian Cell Line

    doi: 10.1155/2022/9664636

    Figure Lengend Snippet: Increasing oxidative stress in cancer by cotreatment of MnP and carboplatin. (a) Intracellular peroxide levels measured by DCF fluorescence probe after 2-hr treatment. PEG-CAT (500 units) were added 24 hr prior to the assay. (b) Intracellular H 2 O 2 levels after 2-hr treatment. (c) Catalase activity after 2-hr treatment. (d) GSH levels were measured by HPLC after 24-hr treatment. (e) MnSOD activity was measured using activity gel with NBT, after 24-hr treatment. (f) GPx activity was determined using a commercial kit after 24-hr treatment. ∗ P ≤ .05 versus hTER7 group. ∗ P ≤ .05 versus no treatment group. # P ≤ .05 versus OV90 group.

    Article Snippet: OV90 human serous ovarian cancer cell lines were purchased from ATCC.

    Techniques: Fluorescence, Activity Assay

    Cotreatment of MnP and carboplatin inhibited mitochondrial respiratory and ATP production in chemoresistant ovarian cancer cells. MnP and/or CBDCA were added to the cells for 6 hr. Live cells were then used for the measurement of OCR and ECAR in OV90 cells (a and c) and in OVCD cells (b and d). (e) Absolute ATP concentration was measured using ATP kits after 24-hr treatment. ∗ P ≤ .05 versus control.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: A Redox-active Mn Porphyrin, MnTnBuOE-2-PyP 5+ , Synergizes with Carboplatin in Treatment of Chemoresistant Ovarian Cell Line

    doi: 10.1155/2022/9664636

    Figure Lengend Snippet: Cotreatment of MnP and carboplatin inhibited mitochondrial respiratory and ATP production in chemoresistant ovarian cancer cells. MnP and/or CBDCA were added to the cells for 6 hr. Live cells were then used for the measurement of OCR and ECAR in OV90 cells (a and c) and in OVCD cells (b and d). (e) Absolute ATP concentration was measured using ATP kits after 24-hr treatment. ∗ P ≤ .05 versus control.

    Article Snippet: OV90 human serous ovarian cancer cell lines were purchased from ATCC.

    Techniques: Concentration Assay, Control

    MnP-mediated oxidative stress inhibited Nrf2 and its downstream target, Trx1 in ovarian cancer carboplatin-resistant cell but not in a normal cell. Cells were cultured for 24 hr prior to MnP treatment (6 hr). Cells were then collected, and RNA was isolated for RT-PCR. (a) Nrf2 and its downstream targets were compared between hTER7, OV90, and OVCD cells. Nrf2 and its downstream targets in (b) hTER7 cell line, (c) OV90 cell line, and (d) OVCD cell line, after MnP treatment. (e) Immunoprecipitation (IP) is performed with antibody to Trx1 and western blot analysis with anti-Keap1, Nrf2, and Trx1 antibodies were subsequently performed. ∗ P ≤ .05 when compared to control.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: A Redox-active Mn Porphyrin, MnTnBuOE-2-PyP 5+ , Synergizes with Carboplatin in Treatment of Chemoresistant Ovarian Cell Line

    doi: 10.1155/2022/9664636

    Figure Lengend Snippet: MnP-mediated oxidative stress inhibited Nrf2 and its downstream target, Trx1 in ovarian cancer carboplatin-resistant cell but not in a normal cell. Cells were cultured for 24 hr prior to MnP treatment (6 hr). Cells were then collected, and RNA was isolated for RT-PCR. (a) Nrf2 and its downstream targets were compared between hTER7, OV90, and OVCD cells. Nrf2 and its downstream targets in (b) hTER7 cell line, (c) OV90 cell line, and (d) OVCD cell line, after MnP treatment. (e) Immunoprecipitation (IP) is performed with antibody to Trx1 and western blot analysis with anti-Keap1, Nrf2, and Trx1 antibodies were subsequently performed. ∗ P ≤ .05 when compared to control.

    Article Snippet: OV90 human serous ovarian cancer cell lines were purchased from ATCC.

    Techniques: Cell Culture, Isolation, Reverse Transcription Polymerase Chain Reaction, Immunoprecipitation, Western Blot, Control